In the last few years, next-generation sequencing (NGS) has demonstrated that it can extract additional information from DNA samples for investigations when current technology fails. When a CE-based STR profile does not produce a hit in traditional databases, NGS capabilities such as higher-plex marker panels, more discriminatory SNP data, and forensic genetic genealogy can provide insights that lead to identifications. The forensic community is recognizing this technology as a viable option for missing persons and unidentified human remains investigations, sexual assault cases, and other violent crimes and are exploring how it can be integrated as more than just a specialty tool. The questions being asked are now focused on the practical aspects of implementation, such as whether this technology is a good financial investment, whether NGS can benefit everyday investigations, and how do you validate. This workshop seeks to share the journey of adopting NGS into their operational casework, and will provide useful information, arguments, case studies, and soft skills as you prepare for your own.

The benefits of next-generation sequencing (NGS) for human identification analysis are increasingly understood but perceived barriers to implementation for general operations still exist. NGS can not only add power to existing processes but also provide unique capabilities for the increasing number of investigations which are beyond the capacity of traditional methods to solve. We will highlight how a range of NGS- based investigative tools can be used to support both mainstream and more advanced analyses for missing persons investigations. This workshop will cover traditional direct comparison DNA analysis with STRs, how to access and use phenotypic and biogeographical information, the different available technologies for forensic investigative genetic genealogy and when to use them, practical workflows that can be implemented into routine laboratory operations, and funding opportunities for NGS.