NEAFS 2020 Annual Meeting Workshops

Public Speaking for Criminal Justice Professionals - A Manner of Speaking

Wednesday, October 14th

9:30 - 12:00

Instructor: Thomas P. Mauriello M.F.S.

“Most people would generally agree that a great deal — probably most — of the presentations

we have to sit through in the business world are awful. They are all too often passionless, boring,and dense with unreadable PowerPoint slides.” 

Whether you speak to one or one-thousand; are comfortable or terrified with public speaking;

whether you have been speaking for many years or just a beginner; "Public Speaking for

Criminal Justice Professionals"; will improve your presentation skills through strategic

planning, preparation, and performance. Techniques for speaking in all settings with confidence,

choosing the right audio-visual technologies, and dealing with questions from an audience, are

explained clearly to help the participant develop their presentation skills. 55 proven effective

presentation tools will be presented, demonstrated, and provided to each participant who attends

this workshop. Knowing your subject does NOT guarantee a successful presentation.

Aristotle, who many recognize as the Father of Public Speaking and Forensic Debate said, “It is

not enough to know what to say, one must know how to say it.” This short four hour workshop

focuses on technique and the recognition that “a speech is composed of three factors — the

speaker, the subject and the listener — and it is to the last of these that its purpose is related.” 


Tom Mauriello created and has presented this workshop to thousands of criminal justice and

counterintelligence professionals throughout the world. Tom is also the host of the webcast, Show. He recently authored the book published by CRC Press, with the

same title as this workshop, “Public Speaking for Criminal Justice Professionals – A

Manner of Speaking”.


So reduce the fear, embarrassment and agony of public speaking; or increase your repertoire of

oral communications skills in the classroom, courtroom or conference room. This training

experience guarantees that you will gain a wealth of knowledge that will “make a difference.”

Analytical Considerations, Requirements and Methodologies for the Opioid Crisis

Wednesday, October 14th

9:30 - 12:00

Topic: Forensic Toxicology

Presentation Title:  Analysis of 17 Fentanyls in Plasma and Blood by UPLC-MS/MS with Interpretation of Findings in Surgical and Postmortem Casework

Duration: 40 minutes

Presenter Name(s) and Title(s): Jonathan P Danaceau, Ph.D, Principal Scientist, Scientific Operations, Waters Corporation


Detection of fentanyl and fentanyl analogs in toxicology laboratories may be underestimated due to limited availability of suitably sensitive and selective assays.  In this presentation, we report on the development and implementation of plasma and blood assays for 17 fentanyls, fentanyl analogs, fentanyl metabolites and synthetic precursors in clinical, and medical examiner, casework.  Studies were performed in postmortem blood obtained in 44 fentanyl-related fatalities and in serial plasma samples from 18 surgical patients receiving intravenous fentanyl therapy while undergoing parathyroidectomy. Multiple synthetic fentanyls were detected with fentanyl, norfentanyl, 4-ANPP and β-OH fentanyl predominating in frequency. In the surgical cases, fentanyl was detected and quantified along with norfentanyl and β-OH fentanyl. The association and relative concentrations of β-OH fentanyl, fentanyl and norfentanyl in the postmortem and clinical studies indicated a metabolic, rather than an illicit, source of β-OH fentanyl.



Topic: Forensic Toxicology

Presentation Title: Detection and Quantitation of Opiates, Opioids, and Stimulants in Whole Blood using SPE and UPLC-MS/MS Analysis 

Duration: 4  0 minutes

Presenter Name(s) and Title(s): Anisha Paul, M.S., Forensic Scientist, Vermont Forensic Laboratory


Increasing DUI drug testing trends in Vermont necessitates a need to develop a method that has the ability to test for narcotic analgesics and stimulants in whole blood. In this presentation, I will talk about the method development and validation for the analysis of Opiates, Opioids, and Stimulants in Whole Blood using Solid Phase Extraction (SPE) and Ultra Performance Liquid Chromatography (UPLC) -Tandem Mass Spectrometry. Once the validation review is completed, this method will be applied in routine blood drug casework analyses for the Vermont Forensic Laboratory. 



Topic: Forensic Chemistry/Seized Materials

Presentation Title: The Power of Knowing NOW with Direct Ionization Mass Spectrometry

Duration: 30 minutes

Presenter Name(s) and Title(s): Khalid Khan, Global Clinical Diagnostics and Forensics, Waters Corporation


Forensic chemistry laboratories handling seized substances are frequently challenged by growing caseloads and are under time pressure to produce results quickly. Waters has developed a new instrument platform called RADIAN ASAP that could potentially improve the workflow and identification of unknowns and alleviate the backlog for seized drug analysis in forensic laboratories. The RADIAN ASAP is a new easy to use, compact system based on Atmospheric pressure Solids Analysis Probe-Mass Spectrometry (ASAP-MS) that enables rapid drug analysis and identification in 60 seconds. Samples are analyzed directly, with minimal sample preparation i.e., following an initial dilution with methanol and subsequent ‘dipping’ of a glass capillary tube into the sample.  To further enhance specificity for drug identification, in source fragmentation is utilized which results in the generation of both precursor and product ions. The resulting data is processed by proprietary LiveID software which facilitates near-real-time matching of acquired data to a spectral library.  The RADIAN-ASAP uses the specificity of mass spectrometry for improved drug identification and enables a rapid triage of samples and depending on the nature of the sample can be considered either a Category A or B technique when following SWGDRUG guidelines.  This presentation will demonstrate the design features of the RADIAN ASAP mass spectrometer system that make it particularly suitable for seized drug analysis and examples of data will be presented from both ‘pure’ drugs and drug mixtures.



Topic: Forensic Chemistry/Seized Materials

Presentation Title: The Use of High-Resolution Mass Spectrometry in Identifying the Likely Causative Agent in an Adverse Reaction to Vaping

Duration: 25 minutes

Presenter Name(s) and Title(s): Scott M. Freeto, MT(ASCP), Principal MS Application Specialist, Clinical Diagnostics and Forensics, Waters Corporation


One of the key features and incentives for the implementation of high resolution, accurate mass spectrometry in the forensic laboratory, is the ability to perform structural elucidation for the identification of novel psychoactive substances and unknown substances. Here we describe an investigation of a suspicious chromatographic peak detected using a single quadrupole mass spectrometer using a quadrupole-time-of-flight (QToF) mass spectrometer and in-silico structural elucidation techniques to identify a probable agent in a vaping solution that resulted in an adverse event in a user of the product.

Forensic Drug Chemistry and the Challenges it Poses: NMS Labs’ Multi-Faceted Approach to Maintain Productivity, Efficiency, and Quality

Wednesday, October 14th

1:30 - 4:00

Speakers: Amanda Andrews, Sarah Shuda, Nicole Lattanzio, Sally Ullman, Aaron Ullman, Sarah Decker, Fran Diamond

The world of forensic drug chemistry is ever changing and always challenging. Each year sees a proliferation of new, more complex compounds, sample types and changes to legislation. It is the challenge to keep up with these changes that all labs face. Productivity and profitability are important factors in any business, but can a lab maintain efficiency and more importantly, quality while meeting these metrics? This workshop is designed to present the multiple approaches NMS labs has adopted to facilitate an efficient means of lab management, sample analysis and reporting in order to provide quality results to clients. Challenges such as sample preparation, data analysis, turn-around-time and managing a multi-site network of labs will be discussed.

Future Trends in Forensic DNA Technology 

Wednesday, October 14th

1:30 - 4:00

A “Uninhibited” video featuring Peterjon McAnany and Nick Andrews- RapidHIT ID

Victim Advocate  - Ashley Spence

RapidHIT ID presentation –  Jon Lucyshyn entitled “Rapid DNA Updates & Case Studies”

A “Uninhibited” video featuring Peterjon McAnany and Nick Andrews - HID Seq Studio

HID Seq Studio presentation- Laura Ascroft entitled “Applied Biosystems™ SeqStudio™ Genetic Analyzer for Human Identification: Evolution of the CE System to Match Your Evolving Needs”


Bio:  Laura Ascroft is a Field Application Scientist at Thermo Fisher Scientific supporting the Northeast territory.  She provides specialized technical applications support and training to ensure customers are successful with the Forensic HID products, services, and workflows.  She also has experience working on the Global HID Professional Services team where she performed technical project management and oversight of global HPS validation projects.  Prior to joining Thermo Fisher, Laura held positions as a Forensic Biologist, Validation Manager, and Supervisor during her 12 year tenure in the Biology Section of the Monroe County Crime Laboratory.

Bio:  Jonathan Lucyshyn is a Field Application Scientist with Thermo Fisher Scientific. Jon joined the team in February of 2020 and is based out of Columbus, Ohio where he resides with my fiancée Jocelyn. Prior to joining Thermo Fisher Scientific, Jon was a Forensic Scientist with the Columbus Police Crime Laboratory in Columbus, Ohio and the Armed Forces DNA Identification Laboratory in Dover, Delaware.  In Jon’s  nine years as a Forensic Scientist, he performed a variety of roles in addition to casework, which included internal validations of several STR chemistries and automated workflows. Additionally, Jon was a Visiting Scientist with the FBI, Counterterrorism Forensic Science Research Unit, optimizing a DNA repair enzyme kit for degraded biological samples and researching microbial biomarkers. Jon has two degrees, a Master of Science in Forensic Science/Forensic Biology from Virginia Commonwealth University and a Bachelor of Science in Biology from James Madison University. Currently, he is on a quest with his fiancée to visit all of the US National Parks visiting 6 out of the total 56 parks in the past two years.

With the proliferation of so many novel psychoactive substances (NPS) and complexity of sample components in seized drug samples, the analysis of these samples requires an innovative approach to incorporate the use of advanced analytical tools, more intense training, and a much greater understanding of the regional legislation. This workshop is designed to present the approach NMS labs has chosen to facilitate an efficient means of sample analysis that provides clients a complete report in a relatively short period of time. Maintaining quality and efficiency at multiple sites is a challenge that will be discussed.